9MHJ
The structure of native Zcp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.511, 92.169, 126.624 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.760 - 1.940 |
| R-factor | 0.195 |
| Rwork | 0.194 |
| R-free | 0.22450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.833 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18rc1_3777: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.760 | 1.990 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 48385 | 3356 |
| <I/σ(I)> | 8.8 | 0.4 |
| Completeness [%] | 99.5 | 94.3 |
| Redundancy | 6.6 | 6.4 |
| CC(1/2) | 0.997 | 0.200 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 8% v/v tacsimate, pH 5.0, and 20% w/v polyethylene glycol 3,350 |
