9MGM

Crystal structure of PRMT5:MEP50 in complex with MTA and compound 24

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	I 2 2 2
Unit cell lengths	100.330, 137.120, 178.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.100 - 2.250
R-factor	0.2481
Rwork	0.246
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.519
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.380	2.310
High resolution limit [Å]	2.250	2.250
Rmerge	0.146	1.025
Rmeas	0.158	1.114
Rpim	0.061	0.434
Total number of observations	385661
Number of reflections	58619	4244
<I/σ(I)>	7.5	1.4
Completeness [%]	100.0
Redundancy	6.6	6.5
CC(1/2)	0.997	0.611

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	283	25% PEG 3350, 0.1 M HEPES (pH 7.5), 0.2 M ammonium sulfate