9MG5
Structure of Saccharomyces cerevisiae mRNA cap (guanine-N7) methyltransferase, Abd1, in complex with sinefungin and GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 63 |
| Unit cell lengths | 95.732, 95.732, 129.256 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.870 - 1.450 |
| R-factor | 0.1177 |
| Rwork | 0.107 |
| R-free | 0.12660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.103 |
| Data reduction software | HKL-2000 |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.870 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.800 | |
| Number of reflections | 183124 | 111696 |
| <I/σ(I)> | 6.3 | 1.7 |
| Completeness [%] | 99.9 | |
| Redundancy | 17.5 | |
| CC(1/2) | 0.990 | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.2 M Ammonium Sulphate, and 20% (w/v) PEG 3350 |
