9MG1
Structure of Kluyveromyces lactis mRNA cap (guanine-N7) methyltransferase, Abd1, in complex with adenine and m7GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.185, 65.235, 74.936 |
| Unit cell angles | 90.00, 94.39, 90.00 |
Refinement procedure
| Resolution | 24.910 - 1.330 |
| R-factor | 0.1864 |
| Rwork | 0.185 |
| R-free | 0.20250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.044 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21rc1_5156)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.910 | 1.340 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Number of reflections | 137174 | 3440 |
| <I/σ(I)> | 3.5 | |
| Completeness [%] | 99.0 | 97.5 |
| Redundancy | 6.7 | |
| CC(1/2) | 0.950 | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 292 | 0.2 M Lithium Sulfate, 25% (w/v) PEG 3350, and 0.1 M Bis-Tris |
