Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-26 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 85.875, 85.875, 350.085 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.320 - 2.750 |
| R-factor | 0.2527 |
| Rwork | 0.250 |
| R-free | 0.30873 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.169 |
| Data reduction software | Aimless |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.320 | 2.880 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.189 | 1.687 |
| Number of reflections | 35391 | 4582 |
| <I/σ(I)> | 15.6 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 24.9 | 25.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Lithium sulfate, 0.1 M MES pH 6.4, 20% w/v PEG 4000 |
