9MFQ

Cat DHX9 in Complex with Compound 10 and ADP

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-11
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	85.730, 85.730, 348.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.900 - 2.990
R-factor	0.22872
Rwork	0.226
R-free	0.27682
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	1.159
Data reduction software	Aimless
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.050	3.170
High resolution limit [Å]	2.990	2.990
Rmerge	0.168	1.186
Number of reflections	27613	4318
<I/σ(I)>	17.9	2.9
Completeness [%]	99.9	99.6
Redundancy	24.2	19.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.15 M Ammonium sulfate, 0.1 M TRIS pH 8, 20% w/v PEG 4000