Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 85.520, 85.520, 350.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.460 - 3.260 |
| R-factor | 0.23815 |
| Rwork | 0.236 |
| R-free | 0.28825 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.156 |
| Data reduction software | Aimless |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.460 | 3.345 |
| High resolution limit [Å] | 3.260 | 3.260 |
| Rmerge | 0.182 | 2.019 |
| Number of reflections | 20314 | 1435 |
| <I/σ(I)> | 15.5 | 2.1 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 25 | 26.4 |
| CC(1/2) | 0.999 | 0.781 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Lithium sulfate, 0.1 M MES pH 6, 20% w/v PEG 4000 |
