9MF1

Crystal structure of RIT1 in the GDP state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-29
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.979110
Spacegroup name	P 2 21 21
Unit cell lengths	35.577, 66.155, 124.717
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.380 - 2.200
R-factor	0.1822
Rwork	0.180
R-free	0.23040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.545
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.380	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.092	0.675
Rpim	0.088	0.652
Number of reflections	15121	3561
<I/σ(I)>	4.7
Completeness [%]	97.8	98.6
Redundancy	2.8
CC(1/2)	0.994	0.727

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	10% w/v PEG 6000, 0.1M Bicine pH 9.0