9MDZ
anti-IL23R VHH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 103 |
| Detector technology | PIXEL |
| Collection date | 2022-09-01 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.00005 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 125.615, 125.615, 41.563 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.720 - 1.270 |
| R-factor | 0.1453 |
| Rwork | 0.145 |
| R-free | 0.15930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.883 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.410 | 1.300 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Rmerge | 0.037 | 0.332 |
| Rmeas | 0.038 | 0.418 |
| Rpim | 0.011 | 0.246 |
| Total number of observations | 456156 | 3080 |
| Number of reflections | 42302 | 1366 |
| <I/σ(I)> | 29 | 1.4 |
| Completeness [%] | 97.2 | |
| Redundancy | 10.8 | 2.3 |
| CC(1/2) | 1.000 | 0.847 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 291 | 800 mM sodium succinate, pH 7 |
