9MDY

16mer self-complementary duplex RNA with s(2)U:A pair sequence 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	99
Detector technology	PIXEL
Collection date	2024-10-30
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97741
Spacegroup name	H 3 2
Unit cell lengths	40.995, 40.995, 122.920
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.973 - 1.360
Rwork	0.186
R-free	0.21750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	2.250
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.380
High resolution limit [Å]	1.360	1.360
Rmerge	0.044	0.493
Rmeas	0.047	0.533
Rpim	0.016	0.201
Number of reflections	8888	405
<I/σ(I)>	49.4	3.4
Completeness [%]	100.0	100
Redundancy	9.2	6.7
CC(1/2)	0.999	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES sodium pH 7.5, 30% v/v Polyethylene glycol 400