9MDX

16mer self-complementary duplex RNA with s(2)U:A pair sequence 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	99
Detector technology	PIXEL
Collection date	2024-10-30
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97741
Spacegroup name	H 3 2
Unit cell lengths	41.381, 41.381, 124.071
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.430 - 1.500
R-factor	0.1904
Rwork	0.188
R-free	0.24090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.977
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.530
High resolution limit [Å]	1.499	1.500
Rmerge	0.057	0.473
Rmeas	0.060	0.500
Rpim	0.020	0.159
Number of reflections	6663	323
<I/σ(I)>	34.6	4.8
Completeness [%]	96.6	95.3
Redundancy	9.5	9.5
CC(1/2)	1.000	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	2.4 M Sodium malonate pH 7.0