9MDW

16mer self-complementary duplex RNA with all Watson-Crick base pairs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	99
Detector technology	PIXEL
Collection date	2024-10-30
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97741
Spacegroup name	H 3 2
Unit cell lengths	41.159, 41.159, 124.195
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.398 - 1.421
Rwork	0.181
R-free	0.22030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.977
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.440
High resolution limit [Å]	1.420	1.420
Rmerge	0.071	0.481
Rmeas	0.076	0.511
Rpim	0.027	0.172
Number of reflections	7975	385
<I/σ(I)>	4.2	4.2
Completeness [%]	100.0	100
Redundancy	9	8.6
CC(1/2)	0.974	0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	20 % v/v Polyethylene glycol 200, 50 mM HEPES pH 7.5, 200 mM Potassium Chloride, 25 mM Magnesium Sulfate