9MCD

Crystal structure of homodimeric Alba from Oryza sativa

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.95372
Spacegroup name	P 61 2 2
Unit cell lengths	71.890, 71.890, 174.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.700 - 2.600
R-factor	0.2102
Rwork	0.208
R-free	0.25970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vm0
RMSD bond length	0.009
RMSD bond angle	0.952
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.940	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.101	0.909
Rmeas	0.111	0.991
Rpim	0.045	0.390
Number of reflections	8854	1042
<I/σ(I)>	14.4	2
Completeness [%]	100.0	100
Redundancy	6.1	6.3
CC(1/2)	0.998	0.736

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	0.2 M SODIUM CACODYLATE pH 7, 0.2 M SODIUM BROMIDE, 23% Peg 5K MME