9MAT
TopBP1 BRCT 7-8 Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97861 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.748, 46.074, 55.948 |
| Unit cell angles | 90.00, 102.53, 90.00 |
Refinement procedure
| Resolution | 47.590 - 1.650 |
| R-factor | 0.1613 |
| Rwork | 0.159 |
| R-free | 0.20740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.995 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.590 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.092 | 0.538 |
| Rmeas | 0.100 | 0.588 |
| Rpim | 0.040 | 0.234 |
| Total number of observations | 183972 | 8906 |
| Number of reflections | 29349 | 1457 |
| <I/σ(I)> | 13.1 | 3.3 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.3 | 6.1 |
| CC(1/2) | 0.996 | 0.816 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.3 M Sodium malonate pH 7.0, 20% w/v PEG 3350 |
