9M9L

The complex structure of Plpro and Frag712

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	82.300, 82.300, 135.340
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.940 - 2.070
R-factor	0.1845
Rwork	0.183
R-free	0.20980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.903
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.070	2.120
High resolution limit [Å]	2.070	2.070
Rmerge	0.195	1.616
Rmeas	0.200	1.658
Rpim	0.045	0.372
Total number of observations	653342	46645
Number of reflections	33015	2381
<I/σ(I)>	13.8	2.5
Completeness [%]	100.0
Redundancy	19.8	19.6
CC(1/2)	0.998	0.543

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	277	1.6 M Sodium Malonate Dibasic Monohydrate