9M9B

The complex structure of Plpro and Frag299

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	83.150, 83.150, 133.590
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.970 - 1.710
R-factor	0.2489
Rwork	0.248
R-free	0.27070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.967
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.970	1.750
High resolution limit [Å]	1.710	1.710
Rmerge	0.264	1.253
Rmeas	0.273	1.355
Rpim	0.067	0.498
Total number of observations	763875	17498
Number of reflections	53611	2559
<I/σ(I)>	6.5	1.9
Completeness [%]	91.7
Redundancy	14.2	6.8
CC(1/2)	0.991	0.513

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	277	1.6 M Sodium Malonate Dibasic Monohydrate