9M9A
The complex structure of Plpro and Frag209
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 84.610, 84.610, 132.390 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.190 - 2.360 |
| R-factor | 0.2003 |
| Rwork | 0.197 |
| R-free | 0.23680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.963 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.190 | 2.420 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.246 | 3.037 |
| Rmeas | 0.252 | 3.119 |
| Rpim | 0.057 | 0.707 |
| Total number of observations | 435911 | 30417 |
| Number of reflections | 22769 | 1579 |
| <I/σ(I)> | 13.2 | 1.1 |
| Completeness [%] | 98.4 | |
| Redundancy | 19.1 | 19.3 |
| CC(1/2) | 0.998 | 0.511 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 277 | 1.6 M Sodium Malonate Dibasic Monohydrate |
