9M99

The complex structure of Plpro and Frag200

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	83.016, 83.016, 132.276
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.090 - 2.280
R-factor	0.2034
Rwork	0.201
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.962
Data reduction software	HKL-3000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.280	2.280
Rmerge	0.163	1.180
Number of reflections	24572	1221
<I/σ(I)>	16.9	1.5
Completeness [%]	99.9
Redundancy	14.2
CC(1/2)	0.988	0.366

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	277	1.6 M Sodium Malonate Dibasic Monohydrate