9M97

The complex structure of Plpro and Frag170

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	82.980, 82.980, 133.220
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.930 - 2.430
R-factor	0.2319
Rwork	0.230
R-free	0.26320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.950
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.930	2.490
High resolution limit [Å]	2.430	2.430
Rmerge	0.286	0.972
Rmeas	0.295	1.000
Rpim	0.069	0.233
Total number of observations	374235	27390
Number of reflections	20316	1505
<I/σ(I)>	9	3.6
Completeness [%]	98.8
Redundancy	18.4	18.2
CC(1/2)	0.997	0.478

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	277	1.6 M Sodium Malonate Dibasic Monohydrate