9M8Z

The complex structure of Plpro and Frag7

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	82.730, 82.730, 133.110
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.820 - 1.840
R-factor	0.2151
Rwork	0.214
R-free	0.24340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.967
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.820	1.890
High resolution limit [Å]	1.840	1.840
Rmerge	0.190	2.232
Rmeas	0.196	2.291
Rpim	0.044	0.511
Total number of observations	897709	67462
Number of reflections	46057	3377
<I/σ(I)>	17	1.6
Completeness [%]	99.3
Redundancy	19.5	20
CC(1/2)	0.999	0.561

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	277	1.6 M Sodium Malonate Dibasic Monohydrate