9M8T

Crystal structure of the ribokinase RBK1 in complex with ADP from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-03
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	I 2 2 2
Unit cell lengths	81.640, 123.150, 168.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.880 - 2.660
R-factor	0.2195
Rwork	0.216
R-free	0.27890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.212
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.820	2.730
High resolution limit [Å]	2.660	2.660
Number of reflections	24435	1771
<I/σ(I)>	15.2
Completeness [%]	98.7
Redundancy	13
CC(1/2)	0.990	0.878

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	8% Tacsimate pH 8.0, 20% PEG 3350, 0.02M Calcium chloride dihydrate