9M86

Crystal structure of SpMETTL16 kinase associated 1 domain in complex with U6 snRNA internal stem loop

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-11
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.978
Spacegroup name	P 1
Unit cell lengths	44.135, 47.136, 61.831
Unit cell angles	87.53, 88.35, 62.87

Refinement procedure

Resolution	33.490 - 2.795
R-factor	0.2086
Rwork	0.206
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.824
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.870
High resolution limit [Å]	2.790	12.490	2.790
Rmerge	0.168	0.062	0.712
Rmeas	0.179	0.067	0.756
Number of reflections	10820	121	772
<I/σ(I)>	12.94
Completeness [%]	98.7
Redundancy	8.7
CC(1/2)	0.995	0.994	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	sodium acetate, sodium cacodylate pH 7.0, Tris-HCl pH 8.5, PEG 8000