9M7T

AcrIE10-DNA complex in Klebsiella pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 62 2 2
Unit cell lengths	117.142, 117.142, 149.464
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.340 - 3.360
Rwork	0.348
R-free	0.35812
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-3000 (7.2.1)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHASER (2.7.0)
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.170	3.540
High resolution limit [Å]	3.358	3.360
Rmerge	0.159	2.354
Number of reflections	9085	1282
<I/σ(I)>	22.6
Completeness [%]	99.6
Redundancy	37
CC(1/2)		0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	300	0.1M HEPES, pH 7.0, 100mM MgCl2, 12% w/v polyethylene glycol 3350