9M7M

Crystal structure of human pseudouridine 5'-monophosphate phosphatase (hHDHD1A) complexed with pseudouridine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-25
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97933
Spacegroup name	P 2 21 21
Unit cell lengths	51.620, 61.090, 68.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.792 - 1.260
R-factor	0.1845
Rwork	0.184
R-free	0.20900
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.551
Data reduction software	XDS (v20230630)
Data scaling software	XDS (v20230630)
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.310
High resolution limit [Å]	1.260	1.260
Number of reflections	107923	6012
<I/σ(I)>	18.89
Completeness [%]	95.6
Redundancy	12.53
CC(1/2)	1.000	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M magnesium chloride, 0.1 M Tris-HCl (pH 8.5), 30% (w/v) PEG 4000