9M7L

Crystal structure of Pseudouridine 5'-monophosphate phosphatase from human (hHDHD1A) in the unliganded state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-05
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97933
Spacegroup name	P 2 21 21
Unit cell lengths	51.560, 60.960, 68.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.384 - 1.360
R-factor	0.149
Rwork	0.149
R-free	0.15750
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.148
Data reduction software	XDS (v20230630)
Data scaling software	XDS (v20230630)
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.410
High resolution limit [Å]	1.360	1.360
Number of reflections	88114	4568
<I/σ(I)>	29.36
Completeness [%]	99.0
Redundancy	12.9
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M magnesium chloride, 0.1 M Tris-HCl (pH 8.5), 30% (w/v) PEG 4000