9M7A
Crystal structure of Serine Acetyltransferase (SAT) from Planctomyces limnophilus in complex with its inhibitor (Cysteine)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU R-AXIS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-01-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5417 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.749, 88.800, 101.400 |
| Unit cell angles | 90.00, 113.22, 90.00 |
Refinement procedure
| Resolution | 46.590 - 2.450 |
| Rwork | 0.239 |
| R-free | 0.28370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.544 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.590 | 2.538 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.050 | 0.226 |
| Number of reflections | 36602 | 36602 |
| <I/σ(I)> | 10.26 | |
| Completeness [%] | 93.5 | 100 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.995 | 0.907 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 278 | 0.02 M Magnesium chloride hexahydrate, 0.05 M PIPES pH7.5, 10% w/v 1,6-hexanediol, 0.001 M spermine |
