9M71
AcrIE10 apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 68.910, 68.910, 233.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.460 - 2.390 |
| Rwork | 0.233 |
| R-free | 0.24920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-3000 (7.2.1) |
| Data scaling software | HKL-3000 (7.2.1) |
| Phasing software | PHASER (2.7.0) |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.460 | 2.450 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.084 | 1.355 |
| Number of reflections | 13895 | 986 |
| <I/σ(I)> | 32.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 35.5 | |
| CC(1/2) | 0.952 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 300 | 4% v/v Tacsimate, pH 7.0, 12% w/v polyethylene glycol 3350 |
