9M70
Crystal Structure of the Carboxyltransferase Subunit of Acetyl-CoA carboxylase from Chloroflexus aurantiacus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-28 |
| Detector | STFC Large Pixel Detector |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 75.210, 112.510, 168.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.770 - 2.780 |
| R-factor | 0.22482 |
| Rwork | 0.222 |
| R-free | 0.27715 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.701 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.19.2) |
| Refinement software | PHENIX (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.770 | 2.879 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Number of reflections | 36628 | 3633 |
| <I/σ(I)> | 5.04 | |
| Completeness [%] | 99.7 | |
| Redundancy | 12.3 | |
| CC(1/2) | 0.984 | 0.447 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.15 M NH4H2PO4, 0.1 M Tris-HCl at pH 8.0, and 50% 2-Methyl-2,4-pentanediol |
