9M6Y

Crystal structure of C1 domain from surface protein SlpM of Lactobacillus brevis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER D8 QUEST
Temperature [K]	150
Detector technology	PIXEL
Collection date	2025-02-22
Detector	Bruker PHOTON III
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	30.065, 37.804, 42.058
Unit cell angles	85.85, 84.92, 66.91

Refinement procedure

Resolution	27.600 - 1.700
R-factor	0.1873
Rwork	0.185
R-free	0.23210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.519
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.600	1.761
High resolution limit [Å]	1.700	1.700
Rmerge	0.233	1.763
Rpim	0.055	0.529
Number of reflections	18676	1890
<I/σ(I)>	11.6	1.91
Completeness [%]	99.7	98.5
Redundancy	19	12
CC(1/2)	0.995	0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M MgCl2 0.1 M Tris pH 8.5 30% PEG 4000