9M6L
Agn1p mutant D237N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-11 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 32 |
| Unit cell lengths | 103.701, 103.701, 101.417 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.790 - 2.100 |
| Rwork | 0.174 |
| R-free | 0.20730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.170 | 2.120 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.073 | 0.692 |
| Number of reflections | 73252 | 4542 |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.1 M Disodium Malonate, 0.1 M HEPES pH7.0, 16 mg/mL protein mixed with 10-fold molar excess of nigero-heptaose |
