9M65
Crystal structure of the pathogen-secreted apoplastic GH12 xyloglucan-specific endoglucanase XEG1
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2024-12-21 |
| Detector | STFC Large Pixel Detector |
| Wavelength(s) | 0.97853 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 139.418, 139.418, 139.418 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.290 - 2.590 |
| R-factor | 0.1956 |
| Rwork | 0.194 |
| R-free | 0.21410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 9ktq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.796 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.290 | 2.680 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rpim | 0.064 | |
| Number of reflections | 14241 | 1414 |
| <I/σ(I)> | 15.9 | |
| Completeness [%] | 99.9 | |
| Redundancy | 20 | |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 5% v/v TacsimateTM pH 7.0, 0.1 M HEPES pH 7.0, 10% w/v Polyethylene glycol monomethyl ether 5,000 |
