9M5B

A Human Fc Receptor Ectodomain in Complex With a Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978610
Spacegroup name	P 1 21 1
Unit cell lengths	57.637, 83.360, 68.588
Unit cell angles	90.00, 109.97, 90.00

Refinement procedure

Resolution	45.420 - 1.660
R-factor	0.1897
Rwork	0.189
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.862
Data reduction software	XDS (2024-07-25)
Phasing software	PHENIX ((1.21.2_5419))
Refinement software	PHENIX ((1.21.2_5419))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.420	45.420	1.760
High resolution limit [Å]	1.660	4.940	1.660
Rmeas	0.068	0.025	1.012
Number of reflections	72255	2792	11574
<I/σ(I)>	19
Completeness [%]	99.8	99.1
Redundancy	6.75
CC(1/2)	0.999	1.000	0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	0.2 M NaCl, 0.1 M BIS-TRIS pH 6.5, 25% w/v polyethylene glycol (PEG) 3,350