9M53
Crystal structure of 1L-myo-inositol 1-phosphate synthase 1 from Oryza sativa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-12-22 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.01200 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.301, 137.079, 217.966 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.450 - 1.790 |
| R-factor | 0.14996 |
| Rwork | 0.148 |
| R-free | 0.18833 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.175 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.080 | 1.820 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.187 | 0.893 |
| Number of reflections | 198484 | 9673 |
| <I/σ(I)> | 10.3 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 7.1 |
| CC(1/2) | 0.993 | 0.652 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Magnesium chloride hexahydrate, 0.1M Tris-HCl at pH8.0 and 25% (w/v) Polyethylene glycol 3,350 |
