9M4R

crystal structure of Arabidopsis thaliana ING1 PHD finger in complex with an H3K4me3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2824
Spacegroup name	P 31 2 1
Unit cell lengths	41.830, 41.830, 69.435
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.226 - 1.700
R-factor	0.1586
Rwork	0.157
R-free	0.19970
Structure solution method	SAD
RMSD bond length	0.019
RMSD bond angle	1.712
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge	0.090	0.075	0.220
Rmeas		0.077	0.227
Rpim		0.018	0.054
Number of reflections	8189	905	813
<I/σ(I)>	11.6
Completeness [%]	99.9	99.8	100
Redundancy	18.2	17.6	17.8
CC(1/2)	0.991	0.997	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	40% PEG400, 5% PEG3000, and 0.1M MES, pH 6.0