9M4G
Structure of AtCDC48 N-domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-19 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980112 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 91.716, 91.716, 242.977 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.250 - 2.600 |
| R-factor | 0.2479 |
| Rwork | 0.244 |
| R-free | 0.31890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.250 | 2.693 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.199 | 3.143 |
| Rmeas | 0.209 | 3.297 |
| Rpim | 0.064 | 0.996 |
| Number of reflections | 12475 | 1486 |
| <I/σ(I)> | 10.4 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.3 | 21 |
| CC(1/2) | 0.998 | 0.308 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 800mM Sodium phosphate monobasic 100mM Potassium phosphate dibasic 100mM Sodium acetate/Acetic Acid |
