Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.539, 38.946, 70.097 |
| Unit cell angles | 90.00, 107.89, 90.00 |
Refinement procedure
| Resolution | 62.370 - 2.010 |
| R-factor | 0.2186 |
| Rwork | 0.217 |
| R-free | 0.24720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.780 |
| Data reduction software | XDS |
| Data scaling software | ADDREF |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.710 | 2.120 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Number of reflections | 22805 | 3291 |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.994 | 0.453 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.1M Sodium citrate tribasic dihydrate, pH5.5,22% polyethylene glycol 1000 |
