9M3K

Crystal structure of GinKR1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97918
Spacegroup name	P 1 2 1
Unit cell lengths	105.464, 66.615, 109.474
Unit cell angles	90.00, 90.24, 90.00

Refinement procedure

Resolution	54.740 - 2.250
R-factor	0.2062
Rwork	0.203
R-free	0.25960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.916
Data scaling software	Aimless (7.1.018)
Phasing software	PHASER (2.7.0)
Refinement software	PHENIX (5.8.0123)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.740	2.250
High resolution limit [Å]	2.250	2.250
Rmerge	0.090	0.090
Number of reflections	72431	46767
<I/σ(I)>	2.25
Completeness [%]	99.9
Redundancy	3.7
CC(1/2)		0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M Ammonium sulfate, 0.1 M Tris pH 8.5, 25% w/v polyethylene glycol 3,350