9M37
Crystal structure of the DgpA2 protein from P581a bound to homoorientin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.660, 45.640, 104.800 |
| Unit cell angles | 90.00, 103.13, 90.00 |
Refinement procedure
| Resolution | 36.740 - 1.900 |
| R-factor | 0.2138 |
| Rwork | 0.212 |
| R-free | 0.24810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.899 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.086 | |
| Number of reflections | 50244 | 3629 |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.6 | |
| CC(1/2) | 0.998 | 0.833 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.18 M magnesium chloride hexahydrate 0.1 M Bis-Tris, pH 5.5 25% w/v PEG3350 |
