9M2T
Crystal structure of glycerol kinase from Entamoeba histolytica complexed with AMP-PNP and glycerol.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.552, 79.675, 204.278 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 1.790 |
| R-factor | 0.19785 |
| Rwork | 0.196 |
| R-free | 0.23732 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.564 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.824 | |
| Rmeas | 0.058 | |
| Rpim | 0.061 | 0.866 |
| Number of reflections | 88486 | 13713 |
| <I/σ(I)> | 23.3 | 3.4 |
| Completeness [%] | 99.4 | 96.6 |
| Redundancy | 10.1 | |
| CC(1/2) | 1.000 | 0.968 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 20 % PEG3350, 0.2 M HCOONa, 50 mM Phosphate buffer (pH 6.8) |
