9M28

Crystal Structure of monomeric Rag-like small GTPase from Asgard Lokiarchaeota (LokiRagM) in complex with GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-14
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.99984
Spacegroup name	P 41 2 2
Unit cell lengths	85.411, 85.411, 151.694
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.410 - 2.000
R-factor	0.19729
Rwork	0.196
R-free	0.22113
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DIALS (3.18.0)
Data scaling software	DIALS (3.18.0)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	151.690	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	36482	1769
<I/σ(I)>	18.8
Completeness [%]	94.4	63.2
Redundancy	21	8
CC(1/2)	1.000	0.180

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292.15	PEG 8000, ethylene glycol, magnesium chloride, calcium chloride