9M26

Crystal structure of Enterobacter cloacae YcdY, a member of the redox enzyme maturation protein family

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2022-10-08
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	65.257, 88.690, 82.250
Unit cell angles	90.00, 103.69, 90.00

Refinement procedure

Resolution	29.850 - 2.500
R-factor	0.2158
Rwork	0.213
R-free	0.26630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.832
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	31272	1530
<I/σ(I)>	12.4	3
Completeness [%]	98.0	98
Redundancy	3.5	3.5
CC(1/2)	0.945	0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 600, calcium acetate, sodium cacodylate