9M22
X-ray structure of human heart fatty acid-binding protein delipidated with Lipidex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.559, 69.915, 33.893 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.230 - 0.800 |
| R-factor | 0.13813 |
| Rwork | 0.138 |
| R-free | 0.14511 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.754 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.010 | 0.810 |
| High resolution limit [Å] | 0.800 | 0.800 |
| Rmerge | 0.073 | 0.429 |
| Number of reflections | 129294 | 4094 |
| <I/σ(I)> | 14.9 | 2.6 |
| Completeness [%] | 94.2 | |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris-HCl (pH8.5), 50% PEG 400 |
