9M1W
Crystal structure of N-prenyltransferase DsKabA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-12-18 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 98.745, 98.745, 206.249 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.890 - 2.850 |
| R-factor | 0.2101 |
| Rwork | 0.203 |
| R-free | 0.27350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.890 | 2.950 |
| High resolution limit [Å] | 2.848 | 2.848 |
| Number of reflections | 21384 | 1387 |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 99.4 | |
| Redundancy | 11.6 | |
| CC(1/2) | 0.959 | 0.841 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | 0.1M Bis-Tris pH 6.5, 20% w/v Polyethylene glycol mono methyl ether 5000 |
