9M1V

Crystal structure of N-terminal domain of hypothetical protein Rv1421 from Mycobacterium tuberculosis H37Rv in complex with uridine diphosphate N-acetyl glucosamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-26
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	50.305, 116.944, 61.646
Unit cell angles	90.00, 93.08, 90.00

Refinement procedure

Resolution	33.010 - 1.700
R-factor	0.1951
Rwork	0.195
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.926
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.121	0.404
Number of reflections	76355	7364
<I/σ(I)>	12.1	2.3
Completeness [%]	98.1
Redundancy	4.8
CC(1/2)	0.992	0.713

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.1M MES monohydrate pH6.0, 8% (v/v) 2-propanol, 26% (w/v) PEG400