9M0U
Structural insights into the tumor suppressor ZMYND11 reveal diverse recognition mechanisms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 198 |
| Detector technology | PIXEL |
| Collection date | 2023-10-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 87.779, 87.779, 129.511 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.300 - 1.890 |
| R-factor | 0.21539 |
| Rwork | 0.214 |
| R-free | 0.23804 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.457 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 54713 | 19198 |
| <I/σ(I)> | 18.8 | |
| Completeness [%] | 84.9 | |
| Redundancy | 8.4 | |
| CC(1/2) | 0.806 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 2 M NH4SO4, 200 mM NaAc,100 mM Hepes pH 7.5,5% MPD |
