9M0G

Crystal structure of human endonuclease G mutant C113A/H141A bound with trans-Resveratrol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2022-06-09
Detector	RAYONIX MX300-HS
Wavelength(s)	0.99984
Spacegroup name	P 21 21 21
Unit cell lengths	64.305, 70.561, 88.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.260 - 3.200
R-factor	0.2079
Rwork	0.198
R-free	0.30060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6njt
RMSD bond length	0.015
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	10.000	3.260
High resolution limit [Å]	3.190	8.000	3.200
Rmerge	0.181	0.118	0.504
Number of reflections	7033	390	330
<I/σ(I)>	7.3
Completeness [%]	99.7	96.5	100
Redundancy	6.8	5.5	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.02 M Citric acid, 0.08 M BIS-TRIS propane / pH 8.0, 16% w/v Polyethylene glycol 3,350