9M01

Crystal structure of the SLA-1*0808 allele and ASFV antigenic peptide at 1.8 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	156.538, 50.080, 53.835
Unit cell angles	90.00, 101.38, 90.00

Refinement procedure

Resolution	47.650 - 1.560
R-factor	0.21037
Rwork	0.209
R-free	0.23832
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.641
Data reduction software	HKL-3000 (7.21)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHASER (2.7.0)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.590
High resolution limit [Å]	1.560	1.560
Rmerge	0.124	1.302
Number of reflections	56188	2867
<I/σ(I)>	14.667
Completeness [%]	96.9
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Sodium thiocyanate,20% w/v Polyethylene glycol 3,350,pH 6.9