9M00
Crystal Structure of the Basal pilin EbpB from Enterococcus faecalis with a partially disordered N-terminal domain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-08 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.87313 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.300, 48.080, 127.010 |
| Unit cell angles | 96.44, 98.04, 90.12 |
Refinement procedure
| Resolution | 43.060 - 2.700 |
| R-factor | 0.24193 |
| Rwork | 0.240 |
| R-free | 0.27918 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.060 | 2.760 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.108 | 0.268 |
| Rpim | 0.079 | 0.193 |
| Number of reflections | 22438 | 1122 |
| <I/σ(I)> | 7.4 | 3.7 |
| Completeness [%] | 89.4 | |
| Redundancy | 2.5 | |
| CC(1/2) | 0.981 | 0.921 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 295 | 100mM SPG buffer pH 9, 25% (w/v) PEG 1500 |
