9LXL

Crystal structure of GH29 family alpha-L-fucosidase from Fusarium proliferatum LE1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17UM
Synchrotron site	SSRF
Beamline	BL17UM
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-11
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	76.757, 76.757, 225.338
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.440 - 2.191
R-factor	0.2036
Rwork	0.202
R-free	0.23390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.480
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.657	2.441
High resolution limit [Å]	2.191	2.191
Rmerge	0.216
Rpim	0.058
Number of reflections	24244	1212
<I/σ(I)>	9.3
Completeness [%]	91.7
Redundancy	14.6
CC(1/2)	0.999	0.589

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	291	0.1M Bis-Tris pH 6.7, 20% PEG 4000