9LXG

Filamin A repeat 21 in complex with LARP4 Ala269-Asn281 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2025-01-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 31 1 2
Unit cell lengths	78.040, 78.040, 32.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.910 - 2.460
R-factor	0.2069
Rwork	0.204
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.014
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.910	2.540
High resolution limit [Å]	2.460	2.460
Number of reflections	4156	405
<I/σ(I)>	21.47	13
Completeness [%]	99.5
Redundancy	17.5
CC(1/2)	0.997	0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.0 M Lithium sulfate, 0.1 M Sodium citrate pH 5.6, 0.5 M Ammonium sulfate